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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
15/09/2021 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RITO, D. S.; VIEIRA, E. F. T.; MENEZES, I. C. de; LAMEIRA, O. A.; POLTRONIERI, M. C.; LEMOS, O. F. de; RODRIGUES, S. de M. |
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Afiliação: |
DANIELLE SANTANA RITO, UFRA; EDUARDO FILIPE TORRES VIEIRA, UFRA; ILMARINA CAMPOS DE MENEZES, CPATU; OSMAR ALVES LAMEIRA, CPATU; MARLI COSTA POLTRONIERI, CPATU; ORIEL FILGUEIRA DE LEMOS, CPATU; SIMONE DE MIRANDA RODRIGUES, CPATU. |
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Título: |
Caracterização morfológica de piperáceas nativas conservadas em casa de vegetação. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Research, Society and Development, v. 10, n. 6, e33410615686, 2021. |
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Idioma: |
Português |
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Conteúdo: |
A família Piperácea possui diversas espécies de interesse econômico, medicinal, ornamental e ambiental. A Embrapa Amazônia Oriental possui uma coleção de piperáceas nativas para garantir a diversidade dessas espécies e o uso em programas de melhoramento genético, dentre outras finalidades. Esse trabalho teve como objetivo descrever as características morfológicas de quatro espécies de piperáceas conservadas em casa de vegetação: Piper marginatum Jacq., Piper montealegreanum Yunck, Piper peltatum L. e Piper tuberculatum Jacq. Foi mensurado o comprimento e largura de folhas, pedúnculo, pecíolo, espigas, entrenós, e altura das plantas. Também foi avaliado o hábito de crescimento, filotaxia das folhas, formato de folhas e caule, presença de lenticelas e ataque de pragas e doenças nos espécimes. Documentou-se a coloração de folhas, caules e espigas usando a carta de Munsell. Os dados consideraram a média das medidas aferidas de quatro espécimes, por espécie, para cada órgão avaliado e comparado a informações da literatura. Os resultados demonstraram que ocorreram diferenças nas características morfológicas das plantas quando cultivadas em ambientes controlados. |
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Palavras-Chave: |
Casa de vegetação. |
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Thesagro: |
Piperaceae; Planta Medicinal. |
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Thesaurus Nal: |
Piper. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226061/1/CaracterizacaoMorfologicaPiperaceasNativas.pdf
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Marc: |
LEADER 01929naa a2200241 a 4500
001 2134448
005 2021-09-15
008 2021 bl uuuu u00u1 u #d
100 1 $aRITO, D. S.
245 $aCaracterização morfológica de piperáceas nativas conservadas em casa de vegetação.$h[electronic resource]
260 $c2021
520 $aA família Piperácea possui diversas espécies de interesse econômico, medicinal, ornamental e ambiental. A Embrapa Amazônia Oriental possui uma coleção de piperáceas nativas para garantir a diversidade dessas espécies e o uso em programas de melhoramento genético, dentre outras finalidades. Esse trabalho teve como objetivo descrever as características morfológicas de quatro espécies de piperáceas conservadas em casa de vegetação: Piper marginatum Jacq., Piper montealegreanum Yunck, Piper peltatum L. e Piper tuberculatum Jacq. Foi mensurado o comprimento e largura de folhas, pedúnculo, pecíolo, espigas, entrenós, e altura das plantas. Também foi avaliado o hábito de crescimento, filotaxia das folhas, formato de folhas e caule, presença de lenticelas e ataque de pragas e doenças nos espécimes. Documentou-se a coloração de folhas, caules e espigas usando a carta de Munsell. Os dados consideraram a média das medidas aferidas de quatro espécimes, por espécie, para cada órgão avaliado e comparado a informações da literatura. Os resultados demonstraram que ocorreram diferenças nas características morfológicas das plantas quando cultivadas em ambientes controlados.
650 $aPiper
650 $aPiperaceae
650 $aPlanta Medicinal
653 $aCasa de vegetação
700 1 $aVIEIRA, E. F. T.
700 1 $aMENEZES, I. C. de
700 1 $aLAMEIRA, O. A.
700 1 $aPOLTRONIERI, M. C.
700 1 $aLEMOS, O. F. de
700 1 $aRODRIGUES, S. de M.
773 $tResearch, Society and Development$gv. 10, n. 6, e33410615686, 2021.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/11/2020 |
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Data da última atualização: |
30/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CÔRTES, M. V. de C. B.; OLIVEIRA, M. I. de S.; MATEUS, J. R.; SELDIN, L.; SILVA-LOBO, V. L.; FREIRE, D. M. G. |
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Afiliação: |
MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MAYTHSULENE INACIO DE SOUSA OLIVEIRA, bolsista CNPAF; JACKELINE ROSSETTI MATEUS, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUCY SELDIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VALACIA LEMES DA SILVA LOBO, CNPAF; DENISE MARIA GUIMARAES FREIRE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
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Título: |
A pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biological Control, v. 152, 104460, 2021. |
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ISSN: |
1049-9644 |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2020.104460 |
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Idioma: |
Inglês |
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Conteúdo: |
Use of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling. MenosUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibit... Mostrar Tudo |
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Thesagro: |
Controle Biológico; Mofo Branco; Patógeno. |
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Thesaurus NAL: |
Biological control; Genetics; Genome; Root rot; Sarocladium oryzae. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 03540naa a2200301 a 4500
001 2126356
005 2021-04-30
008 2021 bl uuuu u00u1 u #d
022 $a1049-9644
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2020.104460$2DOI
100 1 $aCÔRTES, M. V. de C. B.
245 $aA pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens.$h[electronic resource]
260 $c2021
520 $aUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling.
650 $aBiological control
650 $aGenetics
650 $aGenome
650 $aRoot rot
650 $aSarocladium oryzae
650 $aControle Biológico
650 $aMofo Branco
650 $aPatógeno
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aMATEUS, J. R.
700 1 $aSELDIN, L.
700 1 $aSILVA-LOBO, V. L.
700 1 $aFREIRE, D. M. G.
773 $tBiological Control$gv. 152, 104460, 2021.
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Embrapa Arroz e Feijão (CNPAF) |
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